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考えれる1県の原因 c5 to t1

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    5 -Ethynyl-2 -deoxycytidine (EdC) Sigma-Aldrich T511307 Dissolve in DMSO to prepare 40 mM stock, aliquot, and store at -20 C 2 -deoxycytidine (deoxyC) Sigma-Aldrich D3897[jove.com] Prepare 0.5% Triton-X 100 in 1x PBS Click reaction cocktail - Click-iT EdU Alexa Fluor 488 Imaging Kit Molecular Probes C10337 Prepare according to manufactorer's protocol[jove.com] Stocks with titers greater than 1x10 9 PFU/mL work best Sephadex G-25 column (PD-10 Desalting Column) GE Healthcare 17085101 Dimethyl sulfoxide (DMSO) Fisher Scientific D128-1[jove.com]